Single Strand UMI Somatic Variant Calling

July 5, 2017Bioinformatics No Comments

Somatic tumor-only variant calling can be improved by incorporating a UMI per-strand on the source molecule.  You can also perform Duplex Sequencing (see Kennedy et al 2014) but I will go into that in a later post.   Below I will detail the process as well as give you a command line tool that automates all … Read More